Fabrication data of two light-responsive systems to release an antileishmanial drug activated by infrared photothermal heating
Figure 2a: ATR-FTIR spectra of GO, P123 and rGO-P123. Figure 2b: ATR-FTIR spectra of GO, PEI and rGO-PEI. These spectra confirm the obtention of the graphene oxide (GO), and the polymer-functionalized reduced graphene oxide (Pluronic P123, rGO-P123 and Polyethyleneimine, rGO-PEI). Figure 3: Raman spectra of graphite, GO, rGO-P123 and rGO-PEI. The Raman results confirm the presence of the polymers on rGO. Figure 4: PXRD patterns of grafite, GO, rGO-P123 and rGO-PEI. The Powder X-ray diffraction shows the GO was reduced and functionalized. Figures 5 and 6: TEM images of rGO-P123 and rGO-PEI, respectively. TEM images showed a two-dimensional morphology composed of thin, wrinkled and overlapping sheet-like structures of rGO-P123 and rGO-PEI. Figures 7 and 8: SEM images of rGO-P123 and rGO-PEI, respectively. SEM images of rGO-P123 and rGO-PEI revealed smooth, folded and crumpled sheet-like structures. Figures 9a: TGA curves of rGO-P123 and precursors. Figure 9b: TGA curves of rGO-PEI and precursors. From TGA, an amount of 2.43 mg mg-1 of Pluronic® P123 was anchored on the surface of rGO to obtain rGO-P123, while 0.78 mg mg-1 of PEI recovered the rGO surface to yield rGO-PEI. Figure 12: UV-Vis spectra of AmB dispersed in different proportions of PBS:DMSO. These spectra were performed to choose the best mixture of PBS and DMSO to ensure AmB will be used in its monomeric form. Figure 14a: ATR-FTIR spectra of rGO-P123-AmB. Figure 14b: ATR-FTIR spectra of rGO-PEI-AmB. Figures 14 show AmB (amphotericin B) was incorporated in rGO-P123 and rGO-PEI. Figure 15: Phase-contrast optical microscopy images of RAW264.7 macrophages: (a) control and (b) PBS:DMSO (60:40) control and after 72 h of treatment with (c) rGO-PEI 5 µg mL-1 , (d) rGO-P123 5 µg mL-1 , (e) rGO-PEI 15 µg mL-1 , (f) rGO-P123 15 µg mL-1 , (g) rGO-PEI-AmB 5 µg mL-1 and (h) rGO-P123-AmB 5 µg mL-1. Figure 15 shows the rGO-PEI is not toxic to the macrophages. Figure 17a: Absorption spectra of AmB at different concentrations in PBS:DMSO (60:40) obtained at RT. Figure 17b: standard curve of AmB obtained from the absorption spectra using the absorption maximum at λ = 389 nm. This standard curve was used to estimate the amount of AmB loaded and released from rGO-P123-AmB and rGO-PEI-AmB.
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ATR-FTIR spectra were aquired in Thermo Scientific Nicolet iS50 FTIR spectrometer. The spectra were collected in a range of 500–4000 cm-1 , resolution of 4 cm-1 and 64 scans. Raman data were acquired in Witec Alpha 300 system with an excitation green laser equipped with a lens glass of 50x. PXRD data were collected in a Bruker D8 ADVANCE X-ray diffractometer with Cu Kα radiation at room temperature. Origin versions 7.0 and 8.0 were used to analyze the data. TEM data were obtained in a JEOL JEM-1011 microscope with an acceleration voltage of 80 kV. SEM images were obtained using a field emission gun JEOL JSM 7100F microscope with working voltage of 15 kV. TGA analyses were obtained in a Shimadzu TGA-60 from 30 to 600 °C, under N2 and aluminum crucibles. Cary 60 UV-Vis Spectrophotometer (Varian) were used to aquired the spectra showed in Figure 12. UV-Vis spectra were acquired in a quartz cuvette and PBS and DMSO as solvent. Phase-contrast optical microscopy images were aquired in LEICA DMI 6000 microscope.