Meta-Analysis of Hydrogen Therapy on ALI Animal Models

Published: 5 August 2022| Version 3 | DOI: 10.17632/ncpyn2mfsd.3


A growing body of studies has shown that hydrogen therapy is an effective intervention to alleviate Acute Lung Injury (ALI) symptoms in animal models, but no research explores whether different interventions of molecular hydrogen have a discrepancy. Therefore, we used systematic review and meta-analysis to resolve this question through search electric databases of PubMed, EMBASE, Web of Science, and CNKI. 20 studies were selected from the search of 390 studies, and the random-effect model was used to evaluate the effect of Hydrogen therapy by indicators of W/D, PaO2, MDA, SOD, and TNF-α. The quality assessment of All included studies was reviewed by R language. The language differences were verified by SPSS 26.0. Given that hydrogen therapy has different interventions, we used the subgroup analysis to verified that whether 2%H2 and Hydrogen saline have significant differences in the therapy of the animal ALI through STATA 14.0. The results verified that 2%H2 and Hydrogen saline have significant differences in the treatment of ALI animals. Besides, we also used meta-regression to explore the sources of high heterogeneity. Considering species differences, we used species as covariates to find heterogeneity between included studies.


Steps to reproduce

The steps to reproduce were recorded on PROSPERO and manuscript.


Weifang Medical University, Soochow University


Animal, Preclinical Study