Dataset of "Investigating Wolbachia symbiont-mediated host protection against a bacterial pathogen using a natural Wolbachia nuclear insert"
Investigating Wolbachia symbiont-mediated host protection against a bacterial pathogen using a natural Wolbachia nuclear insert Cybèle Prigot-Maurice1*, Baptiste Lheraud1*, Samuel Guéritault1, Sophie Beltran-Bech1, Richard Cordaux1, Jean Peccoud1+ and Christine Braquart-Varnier1+ Material and method part of the article: "We used 196 one year +/- 3-month-old virgin females (89 F-females and 107 NOF-females) in a survival assay to S. enterica infection. Specifically, we applied three different treatments: (1) females not injected at all (17 F-females and 20 NOF-females) to control natural mortality during experiment, (2) females injected with sterile PBS (20 F-females and 26 NOF-females) to control injection effect, and (3) females injected with 8.105 +/- 103 Salmonella enterica typhimurium in 100 nL of sterile PBS (52 F-females and 61 NOF-females). The S. enterica strain, culture, dosage, solution compositions and injection method are described in Prigot-Maurice et al. (2019). The survival assay was performed in five runs, each containing F-females and NOF-females (see supplementary information for details). Following injections, we maintained females in individual boxes with wet soil and rehydrated linden leaves. We monitored their survival rate daily for 7 days (Prigot-Maurice et al., 2019, 2020). "