Osteosarcoma cell density upon 48h incubation with luteolin derivatives

Description

This dataset presents osteosarcoma cell density in the presence of flavonoid derivatives. MG-63, Saos-2, HOS and 143B osteosarcoma cells were incubated with luteolin and structurally-related flavonoids in complete DMEM medium (10% FBS) for 48h. After staining, micrographs were taken at 40x magnification.

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Human osteosarcoma cell lines MG-63, Saos-2, HOS and 143B were cultured in complete DMEM and incubated with humidified atmosphere, 5 % CO2, at 37 ºC. Cells were detached after 4 min incubation with 0.25 % Trypsin/ 1 mM EDTA. Stock solutions included 100 mM flavonoids dissolved in DMSO. For cytotoxicity assays, 5×10(3) cells were seeded per well in 96-well microplates. After overnight adhesion to the plates, culture medium was replaced with medium containing flavonoids at different concentrations (10, 20, 40, 80, 160 micromol/liter) or medium containing DMSO (DMSO control). Cells were incubated for 48 h under culture conditions, the medium was removed and cold 10 % trichloroacetic acid (w/v) was added to each well. After 1-h incubation at 4 ºC, microplates were carefully washed with water and left to dry. Then, fixed cells were incubated for 30 min with 0.05% sulforhodamine B (w/v) dissolved in 1 % acetic acid (v/v). After careful aspiration of sulforhodamine B solution, excess staining was removed by 4 washing steps with 1% acetic acid (v/v) and microplates were left to dry. Micrographs were taken for each condition at 40x magnification (10x ocular lens; 4x objective lens).

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