Raw data of manuscript 'Integrated Microbiota-Bile Acid Analysis as Non-invasive Biomarkers for Ulcerative Colitis Staging Diagnose'
Description
Background: Clinical staging diagnosis and progression tracking for ulcerative colitis (UC) is challenging as poor patient compliance of endoscopic biopsy. Here we perform a study that integrates metabolomic profiling, 16sRNA and metagenomic sequencing on serum and fecal samples from 23 active-state UC patients, 24 remission-state UC patients and 20 healthy volunteers from China, aiming to explore a non-invasive integrative biochemical index to quantitative track and monitor pathological activity of UC. Results: Besides known associations of Fusobacterium nucleatum and Peptostreptococcus stomatis with UC, we found several bile acid-transforming species, including 7α- dehydroxygenase and 7α/β-dehydrogenase expressing microbiota, were significant correlated with UC pathological activity. We identified 7 microbial gene markers that differentiated active and remission-stage UC and healthy control microbiomes. Relevantly, reduced serum deoxycholic acid /cholic acid species ratio and increased fecal ursodeoxycholic acid/chenodeoxycholic acid ratio were associated with pathological activity of UC. Moreover, receiver operating characteristic analysis based on serum/fecal bile acids ratios was much accurate in prediction of active and remission stage outcome. Conclusions: This species-specific temporal change and bile acid dysregulation pattern linked to disease severity indicating that integrated microbiome-bile acid profile maybe implied for disease activity prediction, and that targeting microbiome-mediated restoring gut flora and bile acids homeostasis may be implicative of therapy efficacy. Collectively, these insights will help improve clinical diagnosis and optimize existing medical treatments.