Equivalence between proteins from C. sorokiniana and C. reinhardtii

Published: 4 November 2020| Version 1 | DOI: 10.17632/nksvw4ms57.1
Contributors:
León-Vaz Antonio,
Gotor Cecilia,
Romero Luis,
León Rosa,
Vigara Javier

Description

Chlorella sorokiniana protein sequences in databases, such as KEGG or PANTHER, are not available. The Data include the identification of the Chlamydomonas reinhardtii protein equivalents to the corresponding of Chlorella sorokiniana sequences, which makes possible to use KEGG Database. These data show upregulated and downregulated Chlorella sorokinina proteins, under Cd stress. It can be used in order to perform subsequent proteomic studies in C. sorokiniana trough the protein equivalences with the model microalga Chlamydomonas reinhardtii.

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Chlorella sorokiniana proteins indicated in data were obtained from a proteomic study based on the sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). The data show the differential protein expression between three control cultures of the microalga, cultivated in standard conditions, and three cultures grown in the presence of 250 µM of Cd. The sequence of these C. sorokiniana proteins do not appear in protein databases, such as KEGG and PANTHER. Thus, equivalent proteins of the model green microalga Chlamydomonas reinhardtii were obtained using UniProt database. For this purpose, C. sorokininiana proteins obtained by SWATH-MS were located in Uniprot database. After that, the sequence was selected and aligned using UniProt BLAST. The equivalent proteins for the model microalga Chlamydomonas reinhardtii were selected, after corroborate they had the same function in Chlorella sorokiniana. These data led to realize the correspond analysis in KEGG database in order to study the effect of Cd on Chlorella sorokiniana metabolism. C. sorokiniana 211-32 strain was obtained from the culture collection of the Institute of Plant Biochemistry and Photosynthesis (IBVF; Seville, Spain) and grown mixotrophically in liquid Tris-acetate-phosphate (TAP) medium. The addition of cadmium, as CdCl2, was done before sterilization to a concentration of 250 μM. Cells were harvested by centrifugation at the middle of the exponential phase of growth (40 h), washed and disrupted by sonication. The supernatant obtained was used as protein source. Finally, proteins were precipitated using the TRIzol method for subsequent SWATH-MS analysis. More data are available in PXDO15932 and "Effect of cadmium in the microalga Chlorella sorokiniana: a proteomic study" (References).