Datasets of Survival capacity of the common woodlouse Armadillidium vulgare is improved with a second infection of Salmonella enterica

Published: 12-11-2019| Version 2 | DOI: 10.17632/nn5j83fp34.2
Contributors:
Cybele Prigot-Maurice,
Alexandra Cerqueira de Araujo,
Sylvine Durand,
Tiffany Laverré,
Romain Pigeault,
Julien Verdon,
Philippe Bulet,
Sophie Beltran-Bech,
Christine Braquart-Varnier

Description

Our study aimed to investigate the protective effect confered by immune priming in A. vulgare against Salmonella enterica by determining its setting up and duration. We also analyzed haemocyte variations (concentration and viability) in order to linked them with a potential protection against the bacterial infection. For these purposes, we conducted two distinct experiments on different animals: The first experiment consisted to primed (i.e "vaccinated") animals either with a low dose of living S. enterica bacteria, with LB broth or without injection (control). 1 day , 7 days or 15 days later, we sampled haemolymph of animals and counted haemocytes concentration and viability. The experiment was replicated in 3 independant experimental replicate. Obtained data are available in the file "Prigot_DataHaemocytes". The second experiment was based on the same priming treatment (with low dose of living bacteria, LB broth or nothing). But 1 day, 7 days or 15 days later, we injected a lethal dose of bacteria in all individuals and monitored their survival rates for 7 days. Obtained data are available in the file "Prigot_DataSurvivals". With the two datasets, you will find enclosed a .txt file (Prigot_DataLegends) giving all legends of the datasets colomn heads. Datasets are ready to be used in R program for statistical analysis (the R_script file is also given). Additional informations are available in the published article Prigot-Maurice et al., 2019 (https://doi.org/10.1016/j.jip.2019.107278) For any questions, do not hesitate to contact us.

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Steps to reproduce

Methods and materials used for the collection of these data are stated in the related article "Survival capacity of the common woodlouse Armadillidium vulgare is improved with a second infection of Salmonella enterica" accepted in the Journal of Invertebrate Pathology