Denaturing polyacrylamide gels of RNA cleavage reaction in presence or absence of bacterial GreB

Published: 15-04-2019| Version 1 | DOI: 10.17632/nn7ff7kkw8.1
Contributors:
Moamen Abdelkareem,
Charlotte Saint-André,
Maria Takacs,
Gabor Papai,
Corinne Crucifix,
Xieyang Guo,
Julio Ortiz,
Albert Weixlbaumer

Description

Denaturing polyacrylamide gels of cleavage products show that GreB accelerates RNA cleavage (left). The resulting complex is active and resumes transcription (+NTPs). Using an RNA with phosphorothioate modifications slows down the cleavage without affecting the position (right). To help the reader, cleavage_reaction_a.tif represents the raw data, while cleavage_reaction_b.tif, matches the figure in the publication (Figure S1D).

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