Sample Cone Adaptor for Grinding

Description

Adapter cone for a Foss CT 293 Cyclotec impeller abrasive ring grinder. This funnel is used for faster sample processing and replaces the proprietary glass jar provided with the machine.

Steps to reproduce

1. To print design, download selected open-source 3D files in STL format on Mendeley (see files above). The design choices are either a one or two grooved cone. 2. Obtain proper materials and equipment. For this example, a Form3 SLA Printer (Formlabs MA, USA) and BioMed Clear resin (Catalog # RS-CFG-BMCL-01) (Formlabs MA, USA) was used. The resin was selected for the following properties: high melting temp, high impact resistance, low flexibility, can be sterilized, translucence, and bio-compatibility. 3. Input STL file into a slicer to format the document into something with supports in an orientation that the printer can use. The example used Form Labs 3D slicer. 4. Within the slicing software, rotate the cone model so that the narrow opening is towards the build plate of the printer for best support removal later on. 5. Use the smallest layer setting possible to create a smooth finished product. This example used a 100um setting. 6. Select the smallest raft size the slicer software to reduce material waste. In this example the mini raft setting was used. 7. Save the updated file to the computer. This step will change the file format from the original format to an STL with supports. 8. Upload the formatted file to the 3D Printer. Most printers require the user to select a file that has been uploaded to the printer to execute the job. 9. Print the grinder cone using the print function of the specific printer selected. Do not remove lid as the device is printing to prevent injury from the printing laser. 10. Once the print has been completed, gently remove the cone from the supports wearing gloves. The resin is only biocompatible after curing. 11. Still wearing gloves, soak in 99% isopropyl alcohol for a minimum of 15 minutes to assist in removal of the uncured and loose resin from the print. Isopropyl can be recycled to clean other prints and/or should be disposed of according to proper waste management practices. 12. Wipe down the outside of the print with a paper towel soaked in 99% isopropyl alcohol until there are no drips or excess resin. 13. Put the fresh print in either a UV curing box or UV sterilizer for an hour or until fully cured and no longer tacky at room temperature. The device can now be handled without gloves. 14. Leave fresh print in direct sunlight for 8 hours to off gas and further cure. This product may need up to 48 hours to fully harden before it can be put in an autoclave. This product will NOT over-cure.

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Funding

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