Biomolecules found in Pleistocene fossil in tropical cave: Biofilm characterization by micro Raman spectroscopy.

Published: 22 February 2024| Version 1 | DOI: 10.17632/nscghhnt63.1
Daniel Sousa


The dataset contains 134 raw Raman spectrum from 13 fossil samples from Tayassu and 6 fossil samples from Smillodon. The data set are stored into two folders, named Tayassu and Smillodon. Into these two folders the dataset are organized according the fossil samples. The file title flowing the general principle: the sample number comes first, then, laser line, and the percent of laser bean power used, than the time of sample exposition, than the number of accumulations, the objective used for the measurements, and finally the point number, example: amostra 1 - 785 nm - 0.1% - 10seg - 1 acum - 50X - ponto 1 - cent 1500. The Raman spectrum show of multiple bands between 1200 to 1800 cm-1 associated with organic compounds. Our results show that these organic signatures are linked to biofilm formation, with a high likelihood of biofilm degradation. However, the specific mechanisms involved in the natural degradation of biofilm in fossil records remain unexplored in this study. In our case, the formation of biofilm on fossil bones is attributed to the oligotrophic conditions of the cave sediment matrix. We present a comprehensive model to elucidate the existence of biofilm on fossil bones, emphasizing the pivotal role of post-depositional processes, especially water action, in the cave environment. As the fossils were discovered in a cave setting, post-depositional processes significantly contribute to the formation of the biofilm matrix. Although our study provides insights into biofilm formation, further research is needed to delve into the specific mechanisms driving natural biofilm degradation in fossil records.



Universidade Federal do Vale do Sao Francisco, Universidade Federal de Vicosa, Instituto Federal de Educacao Ciencia e Tecnologia Minas Gerais


Paleontology, Raman Spectroscopy, Fossil Bones


Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco