ACA-21-1395: The data of an all-solid-state acupuncture needle based potentiometric microelectrode for in vivo monitoring of calcium ions in rat cerebrospinal fluid
As a common tool in traditional Chinese medicine, acupuncture needle is an ideal materiel for the development of all-solid-state potentiometric microelectrode for its small tip with micro size. These data contain the details of the fabrication of the calcium ion-selective microelectrode (Ca2+-ISμEs) modified with poly(3,4-ethylenedioxythiophene)-poly(sodium 4-styrenesulfonate) (PEDOT(PSS)) as solid contact. The scanning electron microscope (SEM) images contain the microtopography of the PEDOT(PSS) film. The cyclic voltammetry and electrochemical impedance spectroscopy were carried out for the investigation of the low-frequency capacitance, and the resistance of the microelectrode before and after the modification. The thickness of the solid contact and the calcium ion-selective membrane was investigated by SEM. Potential-time curve of the electrodeposition of the PEDOT/PSS film onto the surface of the microelectrodes under the applied current of 0.5 μA for 200 s was recorded. The condition of the proposed Ca2+-ISμE was optimized through conditioning in different CaCl2 solutions ranged from 1.0 × 10-6 to 3.1 × 10-3 M for different time. The electromotive force measurements were carried out for the investigation of the detection limit, reproductivity, selectivity coefficient against other ions, short- and long-term potential stability. The anti-fouling property of the Ca2+-ISμEs against proteins was investigated through taking BSA as the model protein. The developed Ca2+-ISμEs were used for the in vivo monitoring of the calcium ions in rat cerebrospinal fluid under the stimuli of the spinal cord transection in a living animal.