The complete mitochondrial genomes of Pangasius nasutus and P. conchophilus (Siluriformes: Pangasiidae)

Published: 22 November 2022| Version 1 | DOI: 10.17632/nvpys7jwc5.1
Contributor:
siti amalia aisyah abdul halim

Description

The catfish, Pangasius nasutus and P. conchophilus, are often misidentified between each other due to their similar morphology. Thus, the current study was conducted to differentiate them based on a molecular approach. The complete mitochondrial genomes of P. nasutus and P. conchophilus obtained from the Pahang River (Peninsular Malaysia) were sequenced, assembled, and annotated using Next-generation sequencing (NGS). A 16,465 bp and 16,470 bp length mitogenome sequence of P. nasutus and P. conchophilus, respectively were generated, each containing 13 protein genes, 22 tRNAs, and two rRNAs, typical of most vertebrates. This is the first report of the complete mitochondrial genome sequences of P. nasutus and P. conchophilus. This data is a valuable genetic resource for future studies of these two commercially important species.

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Steps to reproduce

1. Sample collections. 2. DNA extraction. 3. Next Generation Sequencing. 4. Raw data. 5. QC and pre-processing data. 6. De novo assembly. 7. Mitochondrial Annotation. 8. Phylogenetic analysis.

Institutions

Universiti Putra Malaysia Fakulti Pertanian

Categories

Fish, Mitochondrial DNA, Genome, Next Generation Sequencing, DNA Extraction

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