Liver Cell Culture on ITO-SAMs and Metabolite Analysis using NMR for Drug Toxicity Applications Data
Description
NMR spectroscopy provides valuable metabolomic data for liver toxicity screening using HepG2 cells cultured on self-assembled monolayers. This approach offers a comprehensive view of intracellular metabolites, enabling the detection of subtle biochemical changes induced by potential hepatotoxic compounds while focusing on key details like sensitivity, reproducibility and metabolomic profile for drug screening and toxicology applications.
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A Bruker NMR spectrometer, corresponding to a high-resolution proton resonance frequency of 400 MHz at 298K, was employed to acquire the proton NMR Spectra. (Bruker, Billerica, Massachusetts, US). An autosampler (Sample Xpress) was used in this study with all experimental procedures controlled using the software ICON-NMR. 1H water continuous wave irradiation was added during the pre-scan for water suppression. The number of scans taken per sample was 128 scans and the sample measurement took about an hour and a half on average. Proper shimming was also executed before each run.
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Funding
United States Department of Defense
W911NF2120265