The effects of relative humidity and salt concentration on the diameter of hydrophilic polymeric nanofibers obtained by electrospinning: synergistic effect study by Central Composite Design (CCD). Supplementary Material

Description

Results of the 39 experiments of CCD The next micrograph show the experiments of PVA, PVP and PEO fibers obtained by electrospinning technique with and without HAuCl4•3H2O Table 1 and 2 shows. The summary of mean diameter of the fibers obtained by 3 micrograph in three different pints and its respective standard deviations at different Relative Humidity (%)/Temperature (°C) ratio. No fibers were obtained for PVP as show in Figure 3.

Steps to reproduce

Fiber diameter was obtained as follow: Firstly, SEM micrographs of each sample (3 points by replicate for 3 replicates) were obtained, imported into the program, and the scale was properly adjusted (Image > Scale). To facilitate fiber diameter measurement, each micrograph was divided into four quadrants. Depending on the desired number of measurements, 100 measurements were taken per micrograph. The program enhanced the visual quality of the micrographs, improving the sharpness and visibility of the fibers. A filter was applied to enhance image sharpness (Process > Bandpass Filter) before proceeding with measurements (Analyze > Analyze fibers). During this process, the program identified the lines representing the fibers in the micrograph, allowing adjustment of the desired linearity level. Once the program quantified the fibers, results were obtained, including measurements of the area and length of the lines (Results). These data were used to calculate the average size of fibers using a histogram in OriginPro8.

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