CRISPRi is not strand-specific at all loci and redefines the transcriptional landscape

Published: 30 October 2017| Version 2 | DOI: 10.17632/p2652vcdz8.2
Jane Mellor


Fig 1C: qPCR to obtain PCR efficiencies for the clicked versus full-length synthetic DNA oligos (.rex and processed in .xlsx) Fig 1D: Bioscreen data to obtain yeast doubling times Fig 2, 3, 4: Raw phosphorimager files and uncropped scanned X-ray films for HMS2 and GAL1 Northern blots and ethidium bromide-stained rRNA loading Fig 2 - Supplement 2: ChIP-qPCR for (d)Cas9 at two locations in HMS2 (.rex and processed in .xlsx) Fig 3C: Sanger sequencing results for 3'RACE mapping of the GAL1 S transcript (.ab1)