The mitochondrial protease Lonp1 maintains oocyte development and survival by suppressing nuclear translocation of AIFM1 in mammals

Published: 4 November 2021| Version 2 | DOI: 10.17632/p4btc9z6hy.2
Contributor:
flea little

Description

The raw data of SMART RNAseq about MII oocytes from 3-week, 4-week and 5-week control (Lonp1flox/flox) and Lonp1 Gdf9Cre cKO mice and The raw data of SMART RNAseq about oocytes from different stage follicles from Lonp1flox/flox and Lonp1Gdf9Cre cKO mice

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Steps to reproduce

Total oocyte RNA was reverse-transcribed into cDNA and full-length amplified using a Discover-scTM WTA Kit V2 (#N711, Vazyme) according to the manufacturer’s instructions. We purified the amplified cDNA products using Aliquot VAHTS DNA Clean Beads, and quality was assessed with an Agilent 2100 Bioanalyzer. We then used 1 ng of cDNA as starting material for library construction with a TruePrep DNA Library Prep Kit V2 for Illumina® (#TD503, Vazyme). Library sequencing and analysis were performed by GMINIX (Shanghai).

Institutions

Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital, Nanjing University Medical School

Categories

Animal Reproduction, Oocyte Development

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