Raw data supporting the article: Host-pathogen interactome mapping identifies candidate virulence-associated proteins of Mycoplasma hyopneumoniae

Description

This dataset contains the original interaction pairs, protein–protein interaction networks, differential protein lists, enrichment analysis results, and statistical source data supporting the results of the manuscript submitted to Virulence.

Steps to reproduce

Protein–protein interactions between Mycoplasma hyopneumoniae and host proteins were identified using a recombination-based library-versus-library yeast two-hybrid (RLL-Y2H) screening system. Positive clones were collected and subjected to sequencing identification. Interaction pairs were compiled into a dataset and used to construct the interaction network. Functional pathway analysis was performed using a predefined immune-related pathway panel. Experimental validation data were obtained from independent biological replicates and statistically analyzed to generate the figures presented in the manuscript.

Institutions

• Huazhong Agricultural University

  Hubei, Wuhan

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