Co-immunization with two recombinant Newcastle disease viruses expressing ILTV glycoprotein B and H9N2 AIV HA confers cross-protection against three avian pathogens
Description
File 1 : Viral growth kinetics in BHK-21 and CEF cells. The cells were coinfected or individually infected with rTS-HA/rTS-gB at an MOI of 0.01. Viral titers in culture supernatants collected at 12-hour intervals were determined by IFA on the indicated cell lines. File 2: Antibody titers against NDV, AIV and ILTV after immunization, and the date of survival rate, clinical symptoms, tissue viral load and virus shedding after challenging with NDV, AIV and ILTV in SPF chicks File 3: Antibody titers against NDV, AIV and ILTV after immunization, and the date of survival rate, clinical symptoms, tissue viral load and virus shedding after challenging with NDV, AIV and ILTV in commercial chicks File 4: The grouping situation of the test and the data of the virus shedding rate after challenge in SPF or commercial chicks File 5: The original image of Western blot analysis