Effects of voluntary wheel running on hippocampal synaptic remodeling in Rats With Autism Spectrum Disorder Induced by Valproic Acid(raw data)
Description
The hypothesis of this study is that autonomous running wheel exercise can improve valproic acid-induced behavioural deficits such as learning and memory ability, social impairment, novelty cognition in VPA male rats, and mediate the regulation of hippocampal synaptic remodelling through the classical Wnt pathway. The data include Morris water maze, three-box social experiment, new object recognition experiment, Niehl's staining, transmission electron microscopy, qRT-pcr and Western Blotting. tismhrough the data, we can find that the learning memory ability, social ability, novelty cognition of the autistic rats were significantly improved after the exercise intervention; the number of neurons was increased after the exercise, and the synapses were more stable and the synaptic plasticity (number of synapses, synapses, synapses and synapses) was increased. plasticity (synaptic number, synaptic gap, length of prominent active band, thickness of postsynaptic dense matter) were significantly improved; molecular biology experiments showed that the level of Dvl2,GSK3β, β-catenin protein in the classical Wnt pathway changed. It proved that the synaptic dysplasia was improved after exercise, and the over-activation state of classical Wnt pathway was suppressed.TEM and Western Blotting were counted by ImageJ software and then calculated together with the rest of the data by SPSS 23.0.
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Steps to reproduce
The data were obtained through experiments using behavioural, morphological and molecular biological experimental methods.