Methionine-driven methylation modification overcomes plasmid-mediated high-level tigecycline resistance

Published: 20 September 2024| Version 1 | DOI: 10.17632/p6fjgvb2yv.1
Contributor:
丹 房

Description

To explore the impact of tet(X4) expression on bacterial metabolomic state, tet(X4) gene was cloned into a high-copy plasmid, pUC19, to generate pUC19-tet(X4). An empty vector without tet(X4) (pUC19) was included as the control. Subsequently, tet(X4) and pUC19 plasmid was transformed into reference strains E. coli DH5α. Then, we performed metabolomic profiling of tet(X4)-negative and -positive bacteria using Gas Chromatography-Mass Spectrometry (GC-MS).

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Institutions

Yangzhou University

Categories

Antibiotic Resistance

Funding

National Natural Science Foundation of China

32222084 and 32172907

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