Discerning of Antimicrobial peptides (AMPs) from whole genome sequenced tropical and subtropical fruit crops

Published: 09-07-2021| Version 1 | DOI: 10.17632/p6gdn5bg57.1
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Description

Recent outbreaks of infectious diseases caused by multidrug-resistant organisms have served as a stark reminder of the urgent need for novel antimicrobial medicines to protect public health. Anti-microbial peptides (AMPs) have attracted a lot of interest over the last thirty years because of their unique membrane-active antimicrobial mechanism and broad-spectrum activity. These AMPs have a wide range of applications in different fields. It is considered as cationic host defence peptides, anionic antimicrobial peptides/proteins, cationic amphipathic peptides, host defence peptides and alpha-helical antimicrobial peptides. They can protect against a variety of infection-causing organisms, such as bacteria, fungi, parasites, viruses, and tumour cells, and they also have immunomodulatory effects. In the present research, the whole genome sequenced tropical and subtropical fruit crops such as mango, jack, carambola, ber and date palm were selected for the identification of anti-microbial peptides. The whole-genome sequence (WGS) was annotated with the DRAMP database for the retrieval of active anti-microbial peptides. The potential anti-microbial peptides were retrieved in two ways through BLAST and Macrel prediction. Predicted AMPs through both the tools were taken into physicochemical property calculation such as net charge, length of sequence /peptide, hydrophobicity, iso-electric point and amphiphilicity index with the use of the DBAASP database. Based on physicochemical properties calculations the anti-microbial properties were comparatively analyzed and many predicted AMPs had the potential to be utilized against various targets. The study resulted in identifying 44,774 potential AMPs from the selected five tropical and subtropical fruit crops. All the Data from the analysis are stored in the public domain of the Mendeley website. Anyone can access further studies that may result in green technology in controlling diseases both for plants and animals, including human beings.

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