On the Control of Dispersion Interactions Between Biological Membranes and Protein Coated Biointerfaces

Published: 05-02-2021| Version 1 | DOI: 10.17632/pb46knndz2.1
Contributors:
Robert Blackwell,
Arnaud Hemmerle,
Andreas Baer,
Matthias Spät,
Wolfgang Peukert,
Drew Parsons,
Kheya Sengupta,
Ana-Sunčana Smith

Description

This is raw experimental data available for the publication "On the Control of Dispersion Interactions Between Biological Membranes and Protein Coated Biointerfaces", submitted to JCIS. The data consists of raw RICM (reflection interference contrast microscopy) images of giant lamellar vesicles (GUVs) adherend or floating above a protein coated glass substrate. Additionally, scripts (in the form of Jupyter Notebooks, also converted to .html) are supplied to convert these images to vesicle heights. The data is organized as follows (cf. data_organization.txt): - In the folders BSA (and PLL) one can find the code used to convert the normalized RICM movies to height maps in the Jupyter Notebook Convert_to_heignt.ipynb. The notebook has been exported in html as well, and can thus be read with any internet browser. The data in this folder corresponds to the analysis of vesicles on top of a single layer of BSA (or PLL), see paper for details. Each sub-folder contains: - 200ms_cr_Convert2Height_params: the parameters used for the conversion - 200ms_cr_norm.tif: the stack of tif with the RICM intensity normalized following the procedure described in the paper - 200ms_cr_height.tif: the stack of height maps as a tif file, resulting from the execution of Convert_to_height.ipynb - In the folder BSA_NEUTRAVIDIN_BSA one can find three examples of raw RICM movies of vesicles on top of a succesion of BSA, Neutravidin, and BSA layers (see paper for details)

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