Diverse CRISPR-Cas complexes require independent translation of small and large subunits from a single gene

Published: 19-10-2020| Version 2 | DOI: 10.17632/pbkft648ww.2
Contributors:
Tess McBride,
Evan Schwartz,
Abhi Kumar,
David Taylor,
Peter Fineran,
Robert Fagerlund

Description

We demonstrate using biochemical and structural analysis that the type I-D CRISPR-Cas Cascade complex contains Cas11d small subunits expressed from an alternative start site within the cas10d gene. This small subunit is required for I-D Cascade to bind specific DNA targets. We found the alternative expression of the Cas11 small subunit is conserved in types I-D, I-B, and I-C systems, which represent nearly a quarter of sequenced CRISPR-Cas systems. The deposited data corresponds to figures in our manuscript, with the description here indicating the relevant figures.

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