Pleurotus calyptratus (Agaricales, Basidiomycota) - substrate requirements and fruiting dynamics, species-specific primer

Published: 14-04-2021| Version 1 | DOI: 10.17632/pcccgbrfks.1
Oleh Prylutskyi


Supplementary Materals for the paper "Strict substrate requirements alongside rapid substrate turnover may be indicative of an early colonization: a case study of Pleurotus calyptratus (Agaricales, Basidiomycota)" (Prylutskyi et al, 2021). Pleurotus calyptratus is a wood-inhabiting oyster mushroom associated with Populus tremula and P. alba. This fungus is rather common in Ukraine but considered rare in many other European countries. Aiming to find causes for variation in rarity, we conducted habitat mapping and repeated surveys to understand substrate requirements and fruiting dynamics of P. calyptratus. We showed that the fruiting is strictly associated with a tree’s late life and early decay stages, and not with physical properties of a tree trunk like volume or position. This timespan of substrate suitability lasts for 1−3 years, determining rapid substrate turnover. We also detected DNA of P. calyptratus in living tissues of visually healthy trees, meaning its latent development in the hosts. This factor supposedly helps the fungus to pioneer in a fungal succession. Despite the high spatial and temporal variation in fruiting, five annual surveys demonstrated no significant trends in P. calyptratus population’s dynamics, and supported the hypothesis of random variation in fruiting, driven mostly by dynamics of forest stands. Therefore, we hypothesize that P. calyptratus’ relative abundance in Ukraine is explained by continuous substrate supply from large homogenous aspen stands that are rare in the rest of Europe due to intensive management. Data description: SM1 - Full description of species-specific DNA primer development for Pleurotus calyptratus (autors - Olexii Solodiankin, Maryna Kit, Iryna Yatsiuk) SM2 - archive with full Hmsc model for substrate requirements of Pleurotus calyptratus. Data, R-script, fitted models included. Unpack the archive and start with the README.txt. SM3 - archive with full Hmsc model for fruiting spatial and temporal dynamics of Pleurotus calyptratus (2013-2018 years). Data, R-scripts, fitted models and outputs included. Unpack the archive and start with the README.txt. SM4 - full output of the generalized linear model for occupancy of Pleurotus calyptratus


Steps to reproduce

Unpack archives with the models and start with the README.txt. R > 4.0, RStudio IDE recommended.