ACWP-Cassava Mapping Populations Metabolomics
Description
Bi-parental populations of cassava whitefly resistant accession ECU72 crossed with susceptible accessions COL2246 and TMS60444 have been analysed by LC-MS using an untargeted approach. Two F1 families CM8996 (ECU72xCOL2246) and GM8586 (ECU72xTMS60444), plus an F2 family (AM1588) generated by self-crossing a resistant sibling from CM8996, are presented. The dataset include phenotypic classification and LC-MS extracted chemical features. The phenotypic classification of the F1 and F2 bi-parental populations is based on the leaves’ nymph count using the automated system Nymphstar.
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The samples analysed are leaf extracts (50% methanol) spiked in with the internal standard genistein. Samples have been analysed in a 1290 UHPLC- 6560 IM QTOF in ESI negative mode. Chromatographic method was developed using a Zorbax RRHD Eclipse Plus C18 2.1x50 mm, 1.8 μm column and a gradient of 2.5% acetonitrile (solvent A) and acetonitrile (solvent B) modified with 0.03% formic acid. Untargeted LCMS (ESI-QTOF) metabolomics raw datasets. The matrices (.xlsx) were generated by processing raw data files in Profinder v10.0 (Agilent) using the Batch Recursive Molecular Feature Extraction (BRMFE) method.
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Funding
Bill & Melinda Gates Foundation
OPP1200124