Dataset of the first de novo transcriptome assembly of of Typhonium flagelliforme
Published: 27 December 2024| Version 1 | DOI: 10.17632/pdgczjwmhd.1
Contributors:
devit purwoko, , , , , Description
The RNA-seq data was collected from three bulk sections of the leaf of keladi tikus (Typhonium flagelliforme), representing a comprehensive set of transcriptome data from the primary, secondary, and tertiary tuber produced by six-month-old plants.
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Steps to reproduce
Raw reads were submitted to Galaxy (https://usegalaxy.org/) and Trimmomatic 0.38.0 (toolshed.g2.bx.psu.edu/repos/pjbriggs/trimmomatic/trimmomatic/0.38.0) used to trim adapters and low quality bases (slidingwindow, 4 bases, 20 average quality). Paired trimmed reads were submitted to an assembly step via rnaSPAdes 3.15.3 (toolshed.g2.bx.psu.edu/repos/iuc/rnaspades/rnaspades/3.15.3+galaxy2) using default parameters (select k-mer detection option: auto).
Institutions
Badan Riset dan Inovasi Nasional Republik Indonesia
Categories
RNA Sequencing, Transcriptomics, Anticancer Screening, Medicinal and Aromatic Plant Breeding