Alterations in structural and functional responses of bacterial community in response to different levels of compost application in long term fertilized paddy ecosystems
Description
In this study, we used pyro-sequencing tags between V1-V3 regions of the 16S rRNA gene to analyse and compare the effect of long term application of different compost levels on the composition of paddy soil bacterial communities. The treatments were (i) inorganic nitrogen (N), phosphorus (P) and potassium (K) fertilizers (NPK) (ii) NPK and organic compost (C) (NPKC750) (iii) NPKC1500 (iv) NPKC2250 (v) NPKC3000. Inorganic chemical fertilizer was applied as N–P2O5–K2O at a rate of 75-75-75 kg ha-1 from 1954-1970, 100-75-75 kg ha-1 from 1971-1978, 150-86-86 kg ha-1 from 1978-1985 and 110-70-80 kg ha-1 from 1986 onwards. Organic compost was prepared by fermenting rice straws for 5 months and then applied at 7.5, 15.0, 22.5 and 30.0 Mg ha-1 in the NPKC750, NPKC1500, NPKC2250 and NPKC3000 soils, respectively.