Dataset of Coal Bio-gasification and Coalbed Methane Stimulation by Single Well Nutrition Injection in Qinshui Anthracite Coalbed Methane Wells
In-situ coal bio-gasification can be defined as one of the coal bio-mining methodology that fully utilizes the methanogenic bacteria in coal to review the current findings, namely anaerobic digestion of organic components. The following experiment has been done in regards, one vertical well and one multi-branch horizontal well were used as experiment wells and two vertical wells were used as control wells, the pilot test was carried out with single well nutrition injection method. By applying the above mentioned method, the concentration of Cl- ion and number altered in Methanogen spp. were used to trace nutrition diffusion. Furthermore, technical implementation results analysis has been made with the observation of CH4 production changes and coal bed biome evolution. Gas production rates in each well were monitored by using the FLLQ gas roots flow mete. The concentration of CH4 and CO2 were evaluated by using the Agilent 7890A gas chromatograph, on the other hand, concentrations of Cl- were determined by the application of ICS-1100 ion chromatography system. The F420 fluorescence method was adopted to test for the presence of methanogenic bacteria. In the interim of the completion stage, the study stated that the bacterial diversity of underground water of Z-7H well has a high pass sequence with the experimental period of 814 days. Gas production data in Z-159 and Z-7H wells showed the gasification of coal lasted 635 and 799 days, yielded 74817 m3 and 251754 m3 coalbed methane respectively. Furthermore, experimental data presented that one time nutrition injection in anthracite coalbed methane wells achieved an average of 717 days of continuous gas production among all experimental wells. The above fore-said study dedicated the significance of native bacterial fermentation, as it proven the fact that anthracite can be applied to accomplish coal bio-gasification and coalbed methane production stimulation in-situ.
Steps to reproduce
Gas production rates in each well were monitored using FLLQ gas roots flow mete (model: FLLQ, Fuma, Wenzhou, China). The concentration of CH4 and CO2 were analyzed using Agilent 7890A (Agilent, Tokyo, Japan) gas chromatograph. Concentrations of Cl- were identified using an ICS-1100 ion chromatography system (Thermo Scientific Dionex, Bannockburn, United States). The number of methanogens was counted by fluorescence microscope (model: BX41, Olympus, Tokyo, Japan), and the excitation light wavelength was 420 nm (F420 fluorescence). Total genomic DNA was extracted from 1 mL concentrated underground water samples using E.A.N.A. Soil DNA Kit (OMEGA, Georgia, GA, USA). The V4 region of 16S rRNA gene was amplified with polymerase chain reaction (PCR) using primers 515F (5’- GTG CCA GCM GCC GCG GTAA - 3’) and 806R (5’- GGA CTA CHV GGG TWT CTA AT - 3’). 16S rRNA gene libraries were sequenced using an Illumina MiSeq (San Diego, CA, USA) platform and the sequencing data were base-called and demultiplexed using MiSeq Reporter v.1.8.1 (Illumina, SanDiego, CA, USA) with default parameters. The adapter sequences and low quality reads were trimmed away from the raw reads with Trimmomatic v.0.32. The resulting representative sequence set was aligned against the core sequence database of the SILVA 123 release with the Mothur script (www.mothur.org/) and given a taxonomic classification using RDP at the 80% confidence level. Neighbor-joining phylogenetic tree was used to investigate the similarity of species abundance using the Unweighted Pair Group Method with Arithmetic mean (UPGMA) clustering method. Figures were drawn using Origin (OriginPro 2018C).