Data Supporting the Study: Effects of Trelagliptin on Renal Injury in Experimental Diabetic Rats and Ferroptosis-Related Molecular Changes in High-Glucose-Treated Podocytes
Description
This dataset contains the raw and processed data supporting the study entitled "Effects of Trelagliptin on Renal Injury in Experimental Diabetic Rats and Ferroptosis-Related Molecular Changes in High-Glucose-Treated Podocytes". The study investigated the protective effects of trelagliptin (TRE) against diabetic kidney injury and its potential involvement in ferroptosis, oxidative stress, and inflammation. The dataset includes two experimental parts: 1. Animal experiments:** A high-fat diet and streptozotocin (STZ)-induced diabetic rat model was established. Animals were assigned to four groups: Control (Con), Control + TRE (Con+TRE), Diabetes mellitus (DM), and DM + TRE (DM+TRE). Trelagliptin was administered by oral gavage at 40 mg/kg once weekly for 12 consecutive weeks. The collected data include 24-hour urine measurements, urinary protein and creatinine concentrations, kidney-to-body weight ratios, renal histological observations, malondialdehyde (MDA) measurements, and the expression of ferroptosis- and inflammation-related markers. 2. In vitro experiments:** MPC5 podocytes were exposed to high glucose (30 mmol/L) for 24 hours. Four experimental groups were established: MAN, HG+DMSO, HG+Fer-1, and HG+TRE. Ferrostatin-1 (Fer-1) and trelagliptin were used at 1.5 μmol/L with a 2-hour pretreatment. The collected data include reactive oxygen species (ROS) fluorescence measurements and the expression of ferroptosis- and inflammation-related proteins. The dataset contains original experimental images, Western blot data, quantitative PCR results, fluorescence measurements, and corresponding quantitative analyses, as applicable to the uploaded files. These data are provided to support the interpretation, verification, and reproducibility of the findings reported in the associated study.