Full Western blots to accompany manuscript "Nuclear aggregates of NONO/SFPQ and A-to-I edited RNA in Parkinson's disease and Dementia with Lewy Bodies"
Description
Raw western blot data for main and supplemental figures to accompany Belur et al, Neuron, published online May 17th 2024. Please see the publication for details. Dotted line boxes indicate the area used for the figures in the Neuron publication. Cropped out lanes were either due to loading of samples that were not relevant for that particular experiment, or were misloaded on the gel and therefore not representative data. We use a multi-channel western blot detection system that permits simultaneous detection of rabbit and mouse secondary antibodies on the same blot. The channels are indicated in the figure as either 700 or 800, corresponding to excitation wavelength in nanometers. All blots were sequentially probed by multiple antibodies for efficiency purposes, and some images therefore contain residual signals from previous antibody probes. Coomassie gel images refer to the gel that was stained with Coomassie brilliant blue and matches the corresponding transferred blots membranes (shown either to the left or under the western blot).
Files
Steps to reproduce
Please see the detailed methods section in the main Neuron paper