Data for 'Combinatorial Developmental Controls on Striatonigral Circuits'

Published: 04-01-2020| Version 3 | DOI: 10.17632/pst2tzmpc4.3
Contributor:
Ayano Matsushima

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Raw data presented in the paper "Combinatorial Developmental Controls on Striatonigral Circuits" Sharpened tamoxifen-induced birthdating of striatal projection neurons (SPNs) revealed sub-compartmental organization of settlement, i.e., ‘center-surround’ rule. The uploaded data for Figure 2-3 shows the list of distance of individual cells measured from the center and the border of MOR1-positive striosomes. In the Figure 7 of the original paper, striatonigral axons innervating the central striosome-dendron bouquet were demonstrated to be from E12/E13-born SPNs, but not from E11- or E14-born SPNs.

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Data for Figure 2-3 Within the folder ‘Data_distance’ there are five folders containing data for 1. AntStrio; Circular striosome in the anterior striatum (Figs. 2A-B) 2. AntStreak; Subcallosal streak in the anterior striatum (Figs. 2C-D) 3. MidStrio; Circular striosome in the posterior striatum than that shown in Fig. 2 (Figs. 3A-B) 4. Origin; Ventral MOR1-positive zone (Figs. 3C-D) 5. Crym-void; CDG1-negative zone in the tail of caudate nucleus (Figs. 3E-F) The second level of folders divide the data into the following groups; 1. E11; E11.25-born SPNs 2. E12; E12.25-born SPNs 3. E13; E13.25-born SPNs 4. E14; E14.25-born SPNs The third level of folders divide the data for each section. Thus, the number of folders equals, (biological replicate) x (technical replicate) The forth level of folders divide the data for each identified MOR1-positive striosomes (or zones). Each of them containing one cvs file to list the distance from center and border of striosomes measured for each detected tdTomato-positive cell. “Label” column shows the cell ID. There are two rows for each cell. Values in the “Mean” column are the distances from the center (odd number rows) and the border (even number rows) of striosomes. If the tdTomato-positive cell was found outside of striosome, the value 1 was assigned in the “Out” column. Rows with value 2 in the “Out” column are the ones detected by the program, but not verified as cells by eye. Data for Figure 7 Ratio_mCherry_mTagBFP2.xlsx contains the average intensity of mCherry and mTagBFP2 measure at the central striosome-dendron bouquet and the region without mTagBFP2-positive fibers. The latter was used as background intensity to be subtracted. Then the ratio mCherry/mTagBFP2 was calculated for each.