Characterization of entomopathogenic fungi from invasive fall armyworm in China and their risk to native pest.

Description

The concentration of fungus in all fungal treatments is 2×108 spores/ml. The mortality caused by the same fungus isolate was compared by one-way analysis of variance (ANOVA) followed by post hoc Tukey tests with insect species as the factor, the data were checked for normality by Shapiro-Wilk tests before analysis. The data represent the mean of five replicates with standard error. Data with different letters indicate significant differences among different insect species with the same fungal treatment.

Steps to reproduce

The fungal isolates from S. frugiperda were used to compare their pathogenicity against five species of insect pests: S. frugiperda (Lepidoptera: Noctuidae), S. litura (Lepidoptera: Noctuidae), Bemisia tabaci (Hemiptera: Aleyrodidae), Laodelphax striatellus (Hemiptera: Delphacidae) and Nilaparvata lugens (Hemiptera: Delphacidae). Ten newly emerged adults of B. tabaci in a microchamber on tomato plants were sprayed with 2 ml 2×108 spores/ml spore suspension, ten second-instar nymphs of L. striatellus and N. lugens with rice seedlings in a plastic cup were sprayed with 2 ml 2×108 spores/ml spore suspension, and ten second-instar larvae of S. frugiperda and six second-instar larvae of S. litura in six-well tissure culture plate were sprayed with 2 ml 2×108 spores/ml spore suspension and then were fed on an artificial diet. The control insect pests were treated with ddH2O. The treatments on the five insect pest species were all repeated 5 times. All the treatments were maintained in an incubator at 27+2 °C, 50%–70% RH and a 16:8 L:D photoperiod. The survival of the pests was checked every 24 h until no dead samples were found.

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