Data for: Evaluation and Evolutionary Relatedness of 19 Accessions of pepper within Enugu State in South-Eastern Nigeria

Published: 22-12-2018| Version 1 | DOI: 10.17632/pwjj5v6ry9.1
Contributors:
EUGENE OJUA,
Ngozi Abu,
NNAEMEKA EZE

Description

Evaluation and Evolutionary Relatedness of 19 Accessions of pepper within Enugu State in South-Eastern Nigeria. This study assessed the morphologic traits of different varieties of pepper and determined their DNA sequences. Nineteen accessions of pepper were collected from various locations in Enugu State and planted in Botanical garden of the University of Nigeria Nsukka

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The collected fresh fruits were processed and the seeds spread under the sunlight for four days to dry before they were used. This work was conducted in two ways. Firstly the nurseries of the accessions were raised in Botanical garden in nursery baskets on the 18th of August, 2015. The nursery medium was a combination of top soil, river sand and poultry farm manure. The manure was exposed to the air for seven days to allow the evaporation of toxic gases and also to reduce microbial activities that resides in the manure. The seedlings were transplanted after six weeks into polybags for studies. Plant height (cm): Plant height was measured from four randomly selected plants every two weeks starting from three weeks after transplanting. Number of leaves and Number of nodes: These data were collected by numerical count of four randomly selected plants. Length of internodes, mature leaves length and petiole length: These data were measured using a meter ruler and a measuring tape from four randomly selected plants every two weeks starting from three weeks after transplanting. Fruit length: Harvested fruits were opened longitudinally using sharp razor blade and each half measured using a ruler. Fruit width at the widest point: Fruits to be measured were first opened transversely with a sharp knife and the widest points were then measured using a meter rule. Number of fruits per plant: The harvesting started at the ripening of the mature fruits. The number of fruits at each harvest was recorded. At the end of harvest, the total number of fruits/plants was estimated and the mean taken based on the number of sampling plants. Pericarp thickness: This was done by randomly selecting four fruits per plant. The fruits were opened transversely with a sharp knife. The thickness was then measured using a Vernier calliper. The reading was computed by adding both the Vernier scale and main scale of each fruit and the average taken. Single fruit weight: The ripened fruits per plant were plucked after harvest. The weights were then determined using digital weighing balance with a standard reading. The weight of the fruits were recorded in grams. Number of seeds per fruit: The fruits were opened longitudinally with a sharp knife; the seeds in each fruit were emptied into a white paper and counted. DNA Extraction / Isolation was carried out in the Department of Biotechnology, Godfrey Okoye University Enugu. The DNA sequencing was carried out in collaboration with Genewiz, LLC Company, South Plainfield; United State of America.