Data for: Lipidomics data showing different types of dietary fat and fructose interactions on the alteration of hepatic lipid composition in mice

Published: 19 July 2022| Version 1 | DOI: 10.17632/pyzkbm9mry.1
Ming Song


These lipidomics data provide hepatic lipid profile induced by different types of dietary fat and fructose interactions, and are valuable for future studies on different fat sources derived high fat diet-induced nonalcoholic fatty liver disease (NAFLD) mouse models and underlying mechanisms. These lipidomics data showed hepatic fatty acid composition by individual lipid class, including free fatty acid (FFA), diacylglycerol (DAG), triacylglycerol (TAG), phospholipid (PL), and cholesteryl ester (CE). NAFLD is characterized with specific alteration of hepatic lipid composition. Fat and fructose are the two major components of western diet and often consumed together. Excess intake results in NAFLD. We hypothesize that different types of dietary fat in combination with fructose results in differential hepatic lipidomics profile which is associated with distinct metabolic phenotypes. Herein we present hepatic lipidomics data from adult male C57BL/6J mice. The mice were fed with high fat diet (42% of calories derived from fat) enriched with saturated fat (beef tallow), or omega-6 polyunsaturated fat (corn oil) or omega-3 polyunsaturated fat (menhaden fish oil) with or without fructose supplementation through 10% fructose (w/v) in the drinking water ad libitum for 20 weeks. Snap-frozen liver tissues were subjected to lipidomics analysis. Lipids were extracted from liver and individual lipid classes were separated by thin layer chromatography followed by methylation. The methylated fatty acids were extracted and analyzed by gas chromatography (GC).