Direct detection of SARS-CoV-2 using CRISPR-Cas13a and a mobile phone-based device. Fozouni. et al.

Name: Direct detection of SARS-CoV-2 using CRISPR-Cas13a and a mobile phone-based device. Fozouni. et al.
Creator: Parinaz Fozouni
Published: 2020-12-09T15:25:47.110Z
Keywords: Software, Image Analysis

Fozouni, Parinaz; Son, Sungmin; Diaz de Leon Derby, Maria; Knott, Gavin; Gray, Carley; D'Ambrosio, Michael; Zhao, Chunyu; Switz, Neil; Kumar, Renuka; Stephens, Stephanie; Boehm, Daniela; Tsou, Chia-Lin; Shu, Jeffrey; Bhuiya, Abdul; Armstrong, Max; Harris, Andrew; Chen, Pei-Yi; Osterloh, Jeannette; Meyer-Franke, Anke; Joehnk, Bastian; Walcott, Keith; Sil, Anita; Langelier, Charles; Pollard, Katherine; Crawford, Emily; Puschnik, Andreas; Phelps, Maira; Kistler, Amy; DeRisi, Joseph; Doudna, Jennifer; Fletcher, Daniel; Ott, Melanie

doi:10.17632/r3vwyr2w5x.1

Direct detection of SARS-CoV-2 using CRISPR-Cas13a and a mobile phone-based device. Fozouni. et al.

Published: 9 December 2020| Version 1 | DOI: 10.17632/r3vwyr2w5x.1

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We report the development of an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2 from nasal swab RNA that can be read with a mobile phone microscope. The assay achieved ~100 copies/uL sensitivity in under 30 minutes of measurement time and accurately detected pre-extracted RNA from a set of positive clinical samples in under 5 minutes. We combined crRNAs targeting SARS-CoV-2 RNA to improve sensitivity and specificity, and directly quantified viral load using enzyme kinetics. Integrated with a reader device based on a mobile phone, this assay has the potential to enable rapid, low-cost, point-of-care screening for SARS-CoV-2.

Direct detection of SARS-CoV-2 using CRISPR-Cas13a and a mobile phone-based device. Fozouni. et al.

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