Bioautographic determination of antioxidants and carbohydrate hydrolysing enzymes activity of the aqueous cold extract of Adansonia digitata L fruit coupled with UPLC-MS metabolite identifications.
We conducted HPTLC for metabolite derivatization and used bioautography for qualitative estimation of the antioxidant and the carbohydrate hydrolyzing enzyme inhibitory potential of the fruit. Tentative screening for metabolomics was conducted with UPLC-MS.
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Plant A. digitata dry fruit shells were collected from Kordofan state (West Sudan) Chemicals All chemicals used were Sigma Aldrich India products. Appliances: HPTLC Alliance system, United States, and CAMAG TLC scanner IV. 25gr fruit powder was macerated overnight in 500 ml of distilled water filtered and dried over a water bath. Different solvents of varying polarities were tested, was achieved with Toluene: Ethyl acetate: 10% Formic acid, in the ratios of (3:6:1, v/v/v). HPTLC fingerprinting HPTLC system attached to a Diode Array Detector, a gradient pump, and data acquisition software for chromatogram fingerprint generation Bioautography Densitometric scanning was performed under a CAMAG TLC scanner IV, at 254 & 340 nm, 540 and 640nm. photographs were taken with a digital camera. Antioxidant Assay of Adansonia. Digitata L fruit was evaluated qualitatively according to Akar Z. et.al.2017; with DPPH solution. . The procedure was repeated 3 times. α- glucosidase and ß-glucosidase Tests we adopted Simões-Pires et al., 2009 procedure. The experiment was repeated 3 times. 2.4 – Data analysis TLC plates were assessed with bioautography. Chromatograms were generated through software programs acquired within the HPTLC systems. Tentatively identified compounds were read with Mass Lynx V 4.1 software incorporated within the UPLC-Mass spectrometry system and compared with references from the online data bank according to their mass /charge values.