MiRNA and Target Gene Data of Preconditioned Extracellular Vesicles Derived from Umbilical Cord Mesenchymal Stem Cells Treatment In Biliary Atresia Rat Liver

Published: 5 August 2024| Version 1 | DOI: 10.17632/r5y8btj7yt.1
Contributor:
Ratna Puspita

Description

Biliary atresia (BA) is a severe liver disease affecting infants, leading to fibrosis and cirrhosis if untreated. The innovative therapeutic approach using preconditioned extracellular vesicles (EVs) derived from umbilical cord mesenchymal stem cells (UC-MSCs) has shown promise in mitigating liver damage and promoting liver regeneration. The preconditioning of UC-MSCs enhances the therapeutic potential of EVs by enriching them with bioactive molecules, particularly microRNAs (miRNAs). This study aims to characterize the miRNA and target gene profiles of preconditioned EVs derived from UC-MSCs and evaluate their therapeutic effects on BA in a rat model. By understanding the miRNA content and their target genes, we can elucidate the molecular mechanisms through which these EVs exert their hepatoprotective effects. Preconditioned EVs derived from UC-MSCs represent a potent therapeutic strategy for BA. The miRNA and target gene data provide valuable insights into the molecular mechanisms underlying the therapeutic effects, paving the way for further clinical translation and development of EV-based therapies for liver diseases.

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Methods: 1. Isolation and Preconditioning of UC-MSCs: - UC-MSCs are isolated from human umbilical cord tissue and cultured under standard conditions. - Preconditioning involves exposing UC-MSCs to specific stimuli to enhance the secretion of therapeutic EVs. 2. Extraction of EVs: EVs are harvested from the conditioned medium of preconditioned UC-MSCs using ultracentrifugation techniques. 3. Characterization of miRNA Profiles: Nanostring and bioinformatics analysis are employed to identify and quantify the miRNAs present in the EVs. 4. Identification of Target Genes: In silico analysis is used to predict the target genes of the identified miRNAs, focusing on those involved in liver regeneration and fibrosis reduction.

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Stem Cell, Biliary Atresia, Animal Biomedical Model, Biomedical Research, Exosome

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