High-throughput sequencing of Precise CRISPR/Cas9-targeted Recessive Dystrophic Epidermolysis Bullosa immortalised keratinocytes (Ion Torrent)
In this study, the COL7A1 on-target (exon 73) was analysed for precise CRISPR/Cas9 activity. NGS data was assessed in CRISPR/Cas9-treated immortalised patient keratinocytes Despite high on-target activity, high homgeneity of DSB repair outcomes was observed at the nuclease target site. This should result in efficient reframing of the gene, with minimal divergence from the wild-type sequence.
Steps to reproduce
The analysis was designed and performed as previously described (Kocher et al., 2019).