Glycolytic pathway enrichment in T cell subclusters of affected and unaffected dominant dystrophic epidermolysis bullosa and healthy skin

Published: 31 March 2023| Version 1 | DOI: 10.17632/r825m4n3tt.1
Contributors:
Wilson Jr Aala, Ping-Chen Hou, Yi-Kai Hong, Yu-Chen Lin, Yu-Rong Lee, Wei-ting Tu, Maria Papanikolaou, Natashia Benzian-Olsson, Alexandros Onoufriadis, Hans I-Chen Harn, Chen-Peng Chieh, Kurt Lu, John McGrath, Chao-Kai Hsu

Description

Supplemental Figure 1. Glycolysis rate-limiting genes are upregulated in affected dominant dystrophic epidermolysis bullosa-associated GATA3+ Th2 cells. Gene expression of HK1 (hexokinase-1), PKM (pyruvate kinase), and PFKL (phosphofructo-1-kinase isozyme B) were fitted on a kernel density estimation algorithm to estimate probability density using the Nebulosa R package. The black horizontal line in the middle of each box plot indicates the median; the top and bottom borders of the box mark the 75th and 25th percentiles, respectively, and the vertical lines mark the minimum and maximum of the data. Supplemental Figure 2. GATA3+ Th2 cells in affected dominant dystrophic epidermolysis bullosa (DDEB) skin show positive enrichment for glycolysis pathway. Single sample gene set enrichment analysis of T cell subclusters in affected and unaffected DDEB and healthy skin. Normalized enrichment score for canonical glycolysis pathway across clusters is highlighted in the left plot. The black horizontal line in the middle of each box plot indicates the median; the top and bottom borders of the box mark the 75th and 25th percentiles, respectively, and the vertical lines mark the minimum and maximum of the data. In the right plot, a ridge plot is used to visualize the normalized enrichment score across clusters and between groups to show modal scores. Cluster 3 GATA3+ Th2 cells in DDEB affected group shows positive enrichment for canonical glycolysis as shown by positive modal peaks as compared to healthy cells in the same cluster which shows negative modal peaks.

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Institutions

National Cheng Kung University

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Dermatology

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