The regulatory landscape of 5′ UTRs in translational control during zebrafish embryogenesis

Published: 15 November 2024| Version 1 | DOI: 10.17632/rf98wbhzgf.1
Contributors:
,
,
,

Description

The Mendeley Repository folder contains the following: - FIJI macro for automated image analysis (related to figures 2 and 7) - Excel sheets with normalized fluorescence intensity calculations (related to figures 2 and 7) - Updated DaniO5P model weights and contributions - NUPACK structure calculations - All 5' UTR sequences recovered before filtering steps (mentioned in the methods section), which includes 5' UTRs that were not assayed, for the interested reader - Files outputted by next-generation sequencing alignments (deduplicated, mapped reads), used for reporter TPM calculations - The sequence of the plasmid used for cloning the MPRA library Raw imaging data can be found in the Zenodo files: - Zenodo DOI 10.5281/zenodo.13302259 ; related to Figure 2 - Zenodo DOI 10.5281/zenodo.13304863 ; related to Figure 2 - Zenodo DOI 10.5281/zenodo.13304891 ; related to Figure 7 - Zenodo DOI 10.5281/zenodo.13304907 ; related to Figure 7

Files

Steps to reproduce

Information regarding image acquisition and analysis relative to Figures 2 and 7 is detailed in respective excel files (see sheet "info). Information regarding the additional files is provided in the STAR Methods section of the paper.

Institutions

University of Washington, Universitat Basel Department Biozentrum

Categories

Danio Rerio, Embryogenesis, Control of Translation

Licence