Aqueous humor proteomics analyzed by bioinformatics and machine learning in PDR cases versus controls
Published: 2 March 2023| Version 1 | DOI: 10.17632/rfffhrsxs9.1
Contributors:
Hanyi Min, Description
Supplementary data for the study (with raw data)
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Steps to reproduce
The experimental procedure of DIA included two steps. Firstly, a library of spectra was established, including enzymatic digestion following mix samples in equal proportions, liquid phase separation of components, data-dependent acquisition method to collect data, import into Spectronaut software (Navarro et al., 2016) to establish a library of spectra, and set up a DIA scanning method based on the primary ion information. Second, the DIA acquisition of a single sample was performed. The quality control samples, the mixing of multiple samples, was set up to serve as quality control for instrument stability and consistency operations.
Institutions
Chinese Academy of Medical Sciences and Peking Union Medical College
Categories
Proteomics