Raw dataset of seminal proteomic from obesity and diabetes rats

Published: 6 October 2020| Version 5 | DOI: 10.17632/rfp7kfjcsd.5
Contributors:
Marcos Carvalho,
Kelry Mayara Silva,
Viviana Vallejo,
Pablo Ocampo Ortiz,
CRISTIANE SELLA PARANZINI,
null null,
Alessandra Melchert,
Fabiana Souza

Description

Study question: The obesity and diabetes induced in a male rodent can change proteome of spermatozoa? What data shows: These data show the sperm cells profile proteomic of 30 male Wistar rats, separated into 3 groups, control (n = 10), obese (n = 10) and diabetics (n = 09). Rats were fed with commercial food provided controllably to control group and diabetic group or hypercaloric diet added 5% sucrose in ad libitum water to obese group, for 38 weeks. The diabetic group was induced with 35 mg / kg of streptozotocin, 53 days before euthanasia, respecting the spermatogenesis cycle. After euthanasia, sperm were collected and were separated of the seminal plasma by centrifugation and prepared individually for proteomic analysis by ESI Q-Tof mass spectrometer. How the data can be interpreted: There are three folders in the dataset, separated by control, diabetic and obese groups, with all quantified sperm cell proteins, per animal, properly identified.

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Institutions

Centro Nacional de Pesquisa em Energia e Materiais, Universidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina Veterinaria e Zootecnia Campus de Botucatu

Categories

Proteomics, Animal Reproduction, Animal Biotechnology, Rat, Sperm, Metabolism

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