data in brief_γ-Tocotrienol Inhibits Cervical Cancer HeLa Cells Growth through Regulating the PI3K/AKT/mTOR Signaling Pathway

Description

The file is a Data in Brief used by Xu et al study forγ-Tocotrienol Inhibits Cervical Cancer HeLa Cells Growth through Regulating the PI3K/AKT/mTOR Signaling Pathway in vitro. The data showed that γ-T3 (30-60 μM, especially at 45 μM) treatment significantly inhibited HeLa cellular proliferation, and its IC50 was 37.40 ± 5.70 µM at 48 hours. It induced apoptosis and increased the proportion of cells in G0/G1 phase and decreased the proportion at S phase in HeLa cells. The treatment decreased the levels of both phosphorylated and non-phosphorylated (+/-p) mTOR, upstream regulatory factors of PI3K, (+/-p) AKT, downstream regulatory factors of (+/-p) p70S6K, (+/-p) 4E-BP1 and targeted genes of Cyclin D1 and c-Myc related to HeLa cell proliferation and apoptosis. These effects of γ-T3 on the HeLa cells were similar to the actions of Wortmannin (WM), a known blocker of the PI3K/AKT/mTOR signaling pathway. Furthermore, the combination of γ-T3 and WM displayed a strong synergistic effect. These results suggest that γ-T3 could inhibit cell growth by down-regulating the PI3K/AKT/mTOR signaling pathway in human cervical cancer HeLa cells.

Institutions

Categories

Funding

Licence