Electrophysiological and WB data

Published: 8 May 2024| Version 1 | DOI: 10.17632/rk5yfb7xc5.1
Ss C


The hypothesis of this research is that repeated high-frequency visual stimulation (HFS), but not low-frequency visual stimulation (LFS), can lead to a LTP (long-term potentiation)-like increase of visually-evoked field potentials (VEP) in the primary visual cortex (V1), and this potentiation could be caused by more trafficking of membrane AMPA and NMDA receptors. To test this hypothesis, This study examined the effects of repeated HFS (9 Hz) versus LFS (1 Hz) visual stimulation on VEPs and the membrane protein content of AMPA / NMDA receptors in the V1 of cats. We found that repeated HFS caused a long-term improvement in peak-to-peak amplitude (N1P1, P1N2) of V1-cortical VEPs in response to visual stimuli at HFS-stimulated orientation (SO: 90°) and non-stimulated orientation (NSO: 180°), but the effect exhibited variations depending on stimulus orientation: the amplitude increase of VEPs in response to visual stimuli at SO was larger, reached a maximum earlier and lasted longer than at NSO. By contrast, repeated LFS had not significantly affected the amplitude of V1-cortical VEPs in response to visual stimuli at both SO and NSO. Furthermore, the membrane protein content of the key subunit GluA1 of AMPA receptors and main subunit NR1 of AMPA receptors in V1 cortex was significantly increased after HFS but not LFS when compared with that of control cats. These results indicate that HFS can induce LTP-like improvement of VEPs and an increase in membrane protein of AMPA and NMDA receptors in the V1 cortex of cats, which is similar to but less specific to stimulus orientation than the classic LTP. The dataset folder includes two sub-folders: one is for Electrophysiological data, which shows repetitive measures of VEP amplitude in the V1 cortex of each cat (Cat1-4) before and at different time points (0-180 min with an interval of 15 min) after the end of HFS or LFS in response visual stimulus at stimulated orientation (SO) and non-stimulated orientation (NSO). Another sub-folder is for Western blot data, which shows the Western blot bands of the key subunit GluA1 of AMPA receptors and the main subunit NR1 of NMDA receptors as well as β-tubulin (internal reference) in the V1 cortex of 6 cats after HFS, 6 cats after LFS and 6 control cats (CTL). Statistical comparison of VEPs amplitude before and after repeated HFS or LFS as well as the normalized OD of GluA1 and NR1 in the V1 cortex between groups was done using ANOVA and LSD (least significance difference) Post hoc pairwise tests. More detailed descriptions for the electrophysiological data and Western blot data are included in each sub-folder.