Hepatic transcriptomic analysis of swamp eel (Monopterus albus) fed high-carbohydrate diet supplemented with sodium acetate
Description
As the cheapest energy source, the carbohydrate is used in fish feeds to improve physical quality and stimulate protein-sparing action. However, the liver injury usually occurs as the increasing dietary carbohydrate in aquatic animals. Sodium acetate has been reported in studies involved in improving the growth, feed efficiency, immunity and antioxidant in reared fish. Therefore, we hypothesized that high-carbohydrate diet supplemented with sodium acetate can improve high-carbohydrate diet-induced liver injury in fish. The transcriptome of swamp eel (Monopterus albus) was obtained by next generation sequencing (NGS) and de novo assembly. We annotated the unigene of the transcriptome and analyzed differentially expressed genes (DEG) in liver of fish fed the normal diet, high-carbohydrate diet, and high-carbohydrate diet supplemented with 0.2% sodium acetate. Hepatic transcriptomic analysis revealed that several important DEG and KEGG pathway were identified to be potentially involved in ameliorating liver injury of fish.