Data sheet_raw_immunostimulant_SHS
Description
[Rawa data] Fish Oil Extracted from By-Products of Freshwater Fish and its Immunostimulant Activities
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First, the yield and fat contents of all the viscera from pangas catfish, common carp, catfish, snakehead fish, tilapia, and pomfret fish were characterized. Then, all samples (except belly fat from pangas catfish) were extracted using the wet rendering method, which was modified by boiling with distilled water (1:1) at a temperature of 60°C for 30 minutes. The extraction results were filtered to separate the liquids and solids. The liquid was centrifuged at 10,000 rpm to separate the oil and water fractions. The pangas catfish belly fat sample was extracted using the dry rendering method, which was modified by heating in an oven at a temperature of 70°C for 3 hours. These oil samples were then evaluated for their yield, free fatty acid content, peroxide value, p-anisidine value, total oxidation, and fatty acid profile. Two selected samples, tilapia, and pangas catfish were then compared with commercial fish oil in an in vitro study of immunostimulant activity. The immunostimulant activity of fish oil on the proliferation of splenocyte cells was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The procedures involving animals in this study received ethical approval from the Animal Ethics Commission of PT Bimana Indomedical, under the number R.07-20-IR. The separation of single-nucleated blood cells was obtained from the spleen/splenic organ of Balb/c mice and was carried out aseptically. The experiments involving these three samples of fish oil were treated both with and without the addition of ConA, presented in the proliferation rate of the cell. Highly precise data was collected before being analyzed and reported.