Supplementary files to Social network does not influence microbiota of a gregarious bird in a common environment

Published: 16 May 2025| Version 1 | DOI: 10.17632/rrf436g5pm.1
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Description

Data and code to replicate the analyses in the paper "Social network does not influence microbiota of a gregarious bird in a common environment". Silva, Gomes et al. (2025, Behavioral Ecology and Sociobiology). It also contains supplementary material to support results in the published paper. This is composed by one R script file with code to replicate all analyses present in the published paper, one R data file with all data required to run social network analysis, and one zip file containing graphs to support results in the published paper. Specifically, it contains: - 'R script for analyses.R': the complete R scripts needed to replicate all statistical analyses present in the paper. This script contains code to import all data, so no further action, besides running this script, is needed to replicate analyses. - 'R data to social networks.RDATA': contains all data required to run social network analysis. Specifically, it contains the social network, all the individuals data regarding gut and feather microbiome, and further data from RFID antennae required for the analyses. 'R script for analyses.R' provides details on each object contained in this file; main text provides details regarding data collection and quantification. - 'SupMaterial_roostinggraphs': graphs for all differentially abundant bacteria between roosting groups. It contains folders for graphs of feathers (fth) or of cloaca (cl) microbiome, and for amplicon sequence variants (ASV) or genus. Article reference: "Social network does not influence microbiota of a gregarious bird in a common environment", Behavioral Ecology and Sociobiology, 2025. *Sofia Marques Silva, CIBIO/InBIO, Universidade do Porto, Vairão, Portugal; *Ana Cristina R. Gomes, CIBIO/InBIO, Universidade do Porto, Vairão, Portugal; Department of zoology, Stockholm University, Sweden; Gonçalo C. Cardoso, CIBIO/InBIO, Universidade do Porto, Vairão, Portugal. Sandra Trigo, CIBIO/InBIO, Universidade do Porto, Vairão, Portugal; Raquel Xavier, CIBIO/InBIO, Universidade do Porto, Vairão, Portugal. * authors with equal contribution May 2025, Sofia Marques Silva (sofiamarques1@gmail.com) and Ana Cristina R. Gomes (ana.gomes@zoologi.su.se).

Files

Steps to reproduce

'R script for analyses.R' opens in R or R studio and contains all the steps and details to reproduce social network analysis contained in the article. 'R data to social networks.RDATA' needs to be in the same directory so that the 'R script for analyses.R' file runs properly. 'SupMaterial_roostinggraphs' needs to be unzipped to access folders and files inside.

Institutions

Categories

Microbiome, Social Animal Behavior, Social Network Analysis, Gut Microbiome

Funders

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