Augmented secretion of IL-1α from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune-suppressive activity of mesenchymal stromal cells

Published: 15-04-2021| Version 1 | DOI: 10.17632/rrhdsykc5d.1
Contributor:
Nobuo Kondoh

Description

Figure 1. (A) Expression of Il1α mRNA in Sq-1979 cells treated with different serum concentrations. (B) Expression of Il1α mRNA in Sq-1979-1 cells under normoxia or hypoxia. Sq-1979-1 cells were sealed in tedlar bag with normal (20%) or hypoxic (1%) mixed air, seeded in the media containing 10% or 1% FBS for 36h. Figure 2. Expression of IL-1α protein in Sq-1979-1 cells treated with 10% or 1% FBS under normoxic or hypoxic conditions. JPG file for Western blots. Figure 3. (A) Release of IL-1α protein from Sq-1979-1 cells treated with different FBS concentrations. (B) Release of IL-1α protein from Sq-1979-1 cells under normoxia or hypoxia treated with 10% or 1% FBS. Sq-1979-1 cells were seeded in E-MEM supplemented with 10% or 1% FBS under normoxia or hypoxia for 36h. Figure 4. (A) Effects of CMs prepared in lower serum conditions upon the promotion of immune suppressive activities of 10T1/2 cells. (B) Effects of CMs prepared under hypoxia upon the promotion of immune suppressive activities of 10T1/2 cells. Figure 5. (A) Effect of IL-1α removal from CM prepared in low serum upon the promotion of immune suppressive activities of 10T1/2 cells. Relative IFN-γ-producing capability (%) of spleen cells was calculated as described in figure 4 legends. (B) Effect of IL-1α removal from CM prepared in hypoxia upon the promotion of immune suppressive activities of 10T1/2 cells. These are datafiles of above titled manuscript.

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Data in the Excel files for graphics and JPG files for Western blots.