Data for: Meloidogyne incognita (Nematoda: Meloidogynidae) sterol-binding protein Mi-sbp-1 as a target for its management

Published: 26 April 2021| Version 1 | DOI: 10.17632/rt3zvkykhn.1
Contributor:
Uma Rao

Description

These files here are supporting data for the manuscript "Meloidogyne incognita (Nematoda: Meloidogynidae) sterol-binding protein Mi-sbp-1 as a target for its management". Supporting Information Legends Supporting Information 1: Supplementary figures 1 to 4. Supplementary figure 1- Amplification and copy number analysis of M. incognita Mi-sbp-1 gene Supplementary figure 2- The RNAi construct of Mi-sbp-1 for in-planta validation Supplementary figure 3- PCR confirmation of RNAi constructs of Mi-sbp-1 in tobacco transgenic events (T0) Supplementary figure 4- Detection of Mi-sbp-1 expression in T1 tobacco plants by qRT-PCR and effect of HIGS (host induced gene silencing) on transcript abundance of Mi-sbp-1 gene in feeding females. Supporting Information 2: A list of all the differentially expressed genes between dsRNA fed (FD) and starved (ST) nematode J2s as compared to control (FR) J2s, and information on genes of carbon and fat metabolism pathways as determined by the RNA-Seq experiment. The qRT PCR validation of expression of the selected carbon and fat metabolism pathway genes is also provided. Supporting information 3: A list of important pathways differentially expressed between dsRNA fed (FD) and starved (ST) nematode J2s as compared to control (FR) J2s, as identified by the GO-Elite analysis of the RNA-Seq experiment.

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Categories

Bioinformatics, Plant Biotechnology, Parasite, Plant Nematology, Crop Protection

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