Anatomy and physiology of mouse higher visual areas
Physiology: In vitro recordings of neurons in layers 2/3 of higher visual areas in slices of mouse cortex. Single neurons or pairs of neurons are a mix of pyramidal cells and interneurons (parvalbumin and somatostatin). Voltage clamp recordings are EPSCs recorded with optogenetic stimulation of V1 axons in L5 to measure relative strength of feedforward excitation from V1 onto interneurons and pyramidal cells. Current clamp recordings are recorded with current injections to measure intrinsic properties of these neurons. Anatomy: Density measurement of fluorescently labeled V1 axons and cell count density of PV and SOM interneurons in HVAs.